Perfusion deficits may underlie lung and kidney injury in severe COVID-19 disease: insights from a multicenter international cohort study

Background Lung perfusion defects, mainly due to endothelial and coagulation activation, are a key contributor to COVID-19 respiratory failure. COVID-19 patients may also develop acute kidney injury (AKI) because of renal perfusion deficit. We aimed to explore AKI-associated factors and the independent prediction of standardized minute ventilation (MV)—a proxy of alveolar dead space—on AKI onset and persistence in COVID-19 mechanically ventilated patients. Methods This is a multicenter observational cohort study. We enrolled 157 COVID-19 patients requiring mechanical ventilation and intensive care unit (ICU) admission. We collected clinical information, ventilation, and laboratory data. AKI was defined by the 2012 KDIGO guidelines and classified as transient or persistent according to serum creatinine criteria persistence within 48 h. Ordered univariate and multivariate logistic regression analyses were employed to identify variables associated with AKI onset and persistence. Results Among 157 COVID-19 patients on mechanical ventilation, 47% developed AKI: 10% had transient AKI, and 37% had persistent AKI. The degree of hypoxia was not associated with differences in AKI severity. Across increasing severity of AKI groups, despite similar levels of paCO2, we observed an increased MV and standardized MV, a robust proxy of alveolar dead space. After adjusting for other clinical and laboratory covariates, standardized MV remained an independent predictor of AKI development and persistence. d-dimer levels were higher in patients with persistent AKI. Conclusions In critically ill COVID-19 patients with respiratory failure, increased wasted ventilation is independently associated with a greater risk of persistent AKI. These hypothesis-generating findings may suggest that perfusion derangements may link the pathophysiology of both wasted ventilation and acute kidney injury in our population. Supplementary Information The online version contains supplementary material available at 10.1186/s44158-024-00175-1.


Background
Kidney and lung injuries are common and associated with significant morbidity and mortality in critically ill patients [1,2].Patients with acute kidney injury (AKI) are more likely to require mechanical ventilation [1,2], whereas an increased risk for AKI was observed in patients with acute respiratory failure or acute respiratory distress syndrome (ARDS) [3].The co-existence of AKI, even mild or moderate, with ARDS was associated with prolonged ICU and hospital stay, prolonged duration of mechanical ventilation, and higher hospital mortality in a secondary analysis [4] of the LUNG SAFE study [5].The recent COVID-19 pandemic sparked once again the interest about the lung-kidney crosstalk in critically ill patients.Despite initial reports [6], it has been observed that AKI frequency reaches 30% among patients with COVID-19 requiring ICU admission and mechanical ventilation [7,8].
Several mechanisms explain how lung injury may adversely affect kidney function in the context of "typical" ARDS.First, the reduced cardiac output due to positive pressure ventilation [9,10] and/or venous stasis [11] may reduce renal blood flow.In the COVID-19 ARDS, the transmission of the pulmonary pressures to the thoracic compartment and its effect on the renal perfusion may be even more pronounced [12,13], since respiratory system mechanics are often preserved, when compared with typical ARDS [14].Second, the changes in the partial pressure of oxygen and carbon dioxide may affect renal perfusion by influencing vascular resistance [15].Finally, in the context of ventilatory-induced lung injury (VILI), biotrauma may lead to systemic inflammation and organ dysfunction via the release of inflammatory cytokines [16,17].When considering the interplay between COVID-19-related respiratory and kidney failure, unique features of SARS-CoV-2 infection have to be considered.The SARS-CoV-2 virus interaction with the angiotensin-converting enzyme 2 (ACE2) receptor, widely expressed on the renal epithelium, could lead to direct damage to the renal parenchyma [18].Kidney injury could be also systemically mediated by inflammatory cell recruitment, macrophage activation syndrome, and cytokine storm [19].The systemic inflammation promotes the activation of deranged coagulation pathways, leading to perfusion impairment in renal small vessels.Autopsy studies supported the concept of renal "malperfusion, " reporting glomerular and peritubular capillaries thrombosis associated with glomerular ischemia [20,21].In COVID-19 respiratory failure, the presence of perfusion deficits in systemic and pulmonary circulation has been reported [22,23].Additionally, coagulopathy has been identified as an independent predictor for pulmonary and systemic thromboembolic events [22].These findings may suggest a disease-specific AKI characterized by thromboembolic manifestations, coagulation impairment, and endothelial cell swelling with foamy degeneration resulting in endothelialitis [21,22].Interestingly, in COVID-19 respiratory failure, the activation of deranged coagulation, as assessed by increasing levels of d-dimer, was associated with a significant alveolar dead space, resulting in an increased respiratory workload [23].
Whether the presence of dead space in COVID-19 respiratory failure-as result of coagulation activation and ventilation-perfusion mismatch-may explain kidney malperfusion and the development of AKI is yet to be demonstrated.We hypothesize that acute kidney and lung injury may be driven by common and linked pathophysiologic processes including coagulation activation and perfusion deficit.
In this multicenter retrospective study, we aimed to investigate whether laboratory parameters suggestive of inflammation and coagulation activation, along with ventilatory indices indicating the severity of lung injury and increased ventilatory load are associated with AKI development and persistence [24].

Study design and data collection
In this multi-center international retrospective observational study, we enrolled 161 consecutive adult patients, with a diagnosis by real-time PCR of COVID-19 pneumonia requiring mechanical ventilation and ICU admission from February to May 2020.Exclusion criteria were age less than 18 years old and pregnancy.The participating centers included IRCSS Fondazione San Gerardo dei Tintori (Monza, Italy), Policlinico San Marco (Zingonia -Bergamo, Italy), and Galway University Hospitals (Galway, Ireland).This research was part of the STORM Study approved by Istituto Nazionale Malattie Infettive Lazzaro Spallanzani, Rome, Italy (Resolution no.84/2020; NCT04424992).The local ethics committee of Galway University Hospitals, Galway (C.A. 2384), and Policlinico San Marco, Zingonia (Reg.Sperim.N. 118/20), approved the study.Informed consent was waived considering the observational, non-interventional nature of the study.
Demographic, anamnestic, and clinical data of all patients were collected in a dedicated database.Laboratory data, including complete blood count, coagulation panel, liver and kidney function tests, and arterial blood gas analysis, were daily recorded from the day of ICU admission to day 7 and then every 7 days until day 28.Ventilatory settings and ventilatory mechanics parameters were recorded in the same way.The need for adjunctive therapies such as neuromuscular blockade, pronation, inhaled nitric oxide, veno-venous extracorporeal membrane oxygenation (V-V ECMO), and renal replacement therapy (RRT) was recorded.Furthermore, we collected data about ICU outcomes.
We defined and staged AKI by using serum creatinine over 7 days from ICU admission, according to the 2012 Kidney Disease Improving Global Outcomes (KDIGO) clinical practice guidelines [25].In most of the clinical charts, the hourly urinary output was either unavailable or unreliable (e.g., not precisely recorded every hour) due to the pandemic context.For these reasons, we decided to base our AKI definition and classification solely on the creatinine parameter, consistent with literature from the pandemic period [26,27].
Baseline renal function was defined using serum creatinine within six months prior to ICU admission when available.Otherwise, we estimated serum creation by using the following formula, as reported as 2021 CKD-EPI creatinine [28].
where A and B are 0.7 and − 1.2, respectively, if female and 0.9 and − 1.2 if male.Persistent AKI was defined by the continuance of AKI by serum creatinine criteria, according to the KDIGO guidelines, beyond 48 h from its onset.If a complete and sustained reversal of the AKI episode occurred within 48 h, we categorized the AKI as transient [24].Patients with chronic kidney disease (CKD) at any stage were excluded from statistical analysis, considering that serum creatine estimate is not reliable in this population and that one of the most significant risk factors for AKI is pre-existing CKD and AKI itself plays a role in both the CKD development and the progression of pre-existing CKD [29].

Statistical analysis
Normality was assessed using the Shapiro-Wilk test, and continuous data are expressed as mean ± SD or median [IQR], as appropriate.Categorical data are expressed as count (proportion).The test for the trend of normally distributed variables across ordered groups was performed by using linear regression, while the trend of non-normally distributed variables was tested by Jonckheere-Terpstra test.Differences in continuous data were assessed by unpaired Student's T test or U Mann-Whitney test, as appropriate.Categorical data across ordered groups were evaluated via Cochran-Armitage trend test.Chi-square test was performed to compare categorical data between two groups.The effect of the AKI group and the day from the ICU admission on continuous variables was tested using two-way ANOVA.Post hoc analysis was conducted using the Sidak correction method to evaluate the difference in the continuous variable between different AKI groups.The correlations between increasing AKI stages (i.e., No AKI, transient AKI, persistent AKI) and

Study population
We enrolled 161 patients admitted to the intensive care units of the participating centers from February to May 2020.Eighty-nine (55%) patients were admitted to Monza, 51 (32%) at Zingonia, and 21 (13%) at Galway Hospital.We excluded four patients with chronic kidney disease.Thus, 157 patients were included in the final analysis.All patients presented hypoxemic respiratory failure due to COVID-19 requiring ICU admission and invasive mechanical ventilation.We stratified the population into three groups based on the temporary presence and persistence of AKI during the ICU stay.Seventythree (46.5%) patients developed AKI.Of these, 10 (15%) showed a transient AKI, whereas in 58 (37%) patients, the AKI was persistent.We observed more patients without AKI in Monza hospital, while a higher incidence of persistent AKI was reported in Zingonia hospital.No differences in demographic characteristics and main comorbidities were observed among the three groups (Table 1).

Renal characteristics
Baseline creatinine was similar among the three groups.As compared with baseline, a trend toward higher levels of creatinine and urea at ICU admission and higher creatinine peak within 7 days in patients with persistent AKI was observed (Table 1).The levels of creatinine remained higher in the persistent AKI group during the first 7 days after ICU admission (Fig. 1).A higher fluid balance in the first 24 h was observed in the persistent AKI group.Among the patients who developed transient and persistent AKI, 2 (13%) and 16 (28%) patients required continuous renal replacement therapy (CRRT), respectively (Table 1).

Laboratory data at ICU admission
To investigate a possible role of inflammation in the development of AKI, we analyzed inflammatory markers and coagulation function among the three patients' groups.White blood cells were higher in patients who developed AKI compared to those who did not, while inflammatory markers and coagulation function were similar among the three groups (Table 2).During the first 7 days of ICU, patients who developed AKI exhibited higher levels of inflammatory markers and a trend toward higher values of white blood cells.A significant effect of AKI groups on d-dimer level and a trend toward more elevated d-dimer level was observed in the persistent AKI patients (Fig. 1).A more severe metabolic impairment was observed in patients with AKI (Table 2).

Respiratory parameters
Upon admission, the PaO 2 /FiO 2 ratio was similar in patients who developed persistent or transient AKI and in patients who did not.The median of PaO 2 during the The respiratory system mechanics at ICU admission were similar among the groups (Table 2).No difference was observed in arterial CO 2 tension at ICU admission, but a significant trend toward a lower-end tidal CO 2 on admission and during the first week was reported in AKI groups (Table 2).Furthermore, the median dead space, calculated as the difference between PaCO 2 and end-tidal CO 2 divided by PaCO 2 [30], increased significantly across the three cohorts during the first ICU week (Table 2).
On admission and during the first week of ICU, patients presented a higher minute ventilation from no AKI to higher severity classes of AKI (Fig. 2).The proportion of patients who underwent prone positioning decreased from the no-AKI to the persistent AKI group, while no difference in the use of neuromuscular blocking drugs, inhaled nitric oxide and V-V ECMO was observed in the two groups (Table 2).The overall population of patients who underwent prone position was ventilated with higher PEEP levels than those who did not.Patients who underwent prone position in no-AKI and persistent AKI groups at ICU admission (Additional file 1: Table S1)-as well as during the first ICU week (Additional file 1: Table S2)-were exposed to higher PEEP levels than those who did not.All data represent mean ± SEM. $ refers to the effect of AKI groups on the continuous variable; $$ refers to the effect of day from ICU admission on the continuous variable; $$$ refers to the interaction between AKI groups and day from ICU admission on the continuous variable.Upon dividing AKI subgroups into ordinal quartiles of respiratory load indicators, we noted a significantly higher percentage of subjects developing persistent AKI in the higher MV and standardized MV quartiles and a trend toward a higher percentage of persistent AKI in the quartiles with higher PaCO 2 (Fig. 3).We did not observe any difference in the percentage of persistent AKI patients among the PaO 2 /FiO 2 ratio quartiles (Fig. 4).
To assess whether standardized MV [5], as an index of the ventilatory load needed to keep PaCO 2 within the normal range, may independently affect AKI incidence and persistence in severe COVID-19 patients, we performed an ordered multivariable analysis.After adjusting for confounders, including non-respiratory SOFA score, bicarbonate level at ICU admission, prone position, and admission center, standardized MV remained independently associated with a higher risk to develop AKI and AKI persistence (Table 3).

Hemodynamic parameters
We examined mean arterial pressure, heart rate, central venous pressure, vasopressor requirement, and lactate levels during the first ICU week in our population to evaluate the relationship between hemodynamics and renal function.A trend toward a more severe hemodynamic impairment was observed in patients with AKI.Patients with persistent AKI presented higher lactates, heart rate, and cardiovascular SOFA scores.No  difference was reported in mean arterial pressure and central venous pressure among the groups (Table 2).

Outcomes
A similar incidence of thromboembolic complications (i.e., stroke, venous thromboembolism (VTE), pulmonary thromboembolism (PTE)) among the groups was found.Patients without AKI show a higher rate of bacterial over-infection during their ICU stay.Patients with persistent AKI show shorter duration of both invasive and non-invasive ventilation and shorter ICU and hospital length of stay.A significant tendency to higher ICU mortality was observed by moving through the AKI spectrum (Table 2).No difference in renal recovery was described when comparing transient AKI and persistent AKI classes (Table 2).

Discussion
The main results of this study can be summarized as follows: • Among 157 COVID-19 patients on mechanical ventilation, 47% developed AKI: 10% had transient AKI, and 37% had persistent AKI.• Across increasing severity of AKI groups, despite similar levels of paCO 2 , we observed an increased minute ventilation and a higher standardized minute ventilation, a robust proxy of dead space.• The degree of hypoxia was not associated with differences in AKI severity.• After adjusting for other clinical and laboratory covariates, standardized minute ventilation remained an independent predictor of AKI development and persistence.
• d-dimer levels were increased early after ICU admission only in the persistent AKI group.
Among mechanically ventilated COVID-19 patients admitted to the ICU, AKI is a common yet severe complication.In our cohort, we observed a 46.5% incidence of AKI.Similar results were found in a recent study testing AKI incidence in patients with severe COVID-19, they observed a 53% AKI incidence in their patients [7].In severe COVID-19 patients, a higher AKI incidence than in "typical" ARDS has been reported [3,4].Independent risk factors for AKI in non-COVID-19 ARDS include sepsis, non-cardiogenic shock, transfusion-related acute lung injury, and pancreatitis [31,32], which were absent or extremely rare in our population.Therefore, other factors must be examined as potential causes of AKI in our cohort.We acknowledge that in emergency settings, such as during the initial European wave of COVID-19, the impracticality of maintaining a high standard of care may lead to severe respiratory distress, dehydration, and hypovolemia, potentially resulting in more severe kidney failure.Patients with persistent AKI showed to have higher serum creatinine and urea at ICU admission and a more positive fluid balance during the first 24 h, underling the severity of the organ failure in this group.Furthermore, in the persistent AKI group, we observed a higher percentage of patients with KDIGO stage 3 AKI, which has been associated with an increased risk of mortality in a COVID-19-related ARDS [29].The rapid shortage of ICU beds was particularly important in small centers [6].The surge of patients admitted to the hospital and the lack of ICU beds may be the reason for a higher incidence of AKI in Zingonia Hospital, a peripheric center very close to the Italian pandemic epicenter when compared with Monza and Galway hospitals.
We decided to stratify our patients according to the ADQI definition of transient and persistent AKI [24], considering the clinical relevance of this recent classification.When considering our cohort of AKI patients, 10 (21%) subjects developed transient AKI, while 58 (79%) had persistent AKI.In a retrospective analysis of all-cause ICU patients, the incidence of transient and persistent AKI was 73.4 and 16.5%, respectively [33].Conversely, in septic patients, the incidence of transient and persistent AKI was 18.4% and 81.6%, and sustained AKI was found to be independently associated with sepsis mortality, as well as inflammatory and procoagulant responses [34].In our study, COVID-19-related AKI in ICU was frequent and persistent.This finding is consistent with previous literature [35] and may suggest some overlapping mechanisms with sepsis-related AKI.We observed a significant trend toward higher ICU mortality in the persistent AKI group, in line with a previous report, where persistent AKI has been correlated with higher progression to CKD, morbidity, and mortality [36].When analyzing inflammatory biomarkers, white blood cell count at ICU admission showed a higher trend in persistent AKI patients at ICU admission and during the first 3 days of ICU.Despite similar serum procalcitonin (PCT) at ICU admission, higher PCT levels were found during the first ICU week.Prolonged persistence of plasma PCT should include both the possibility of a sustained production and an impaired renal clearance of procalcitonin in the context of AKI [37].Unexpectedly, a lower rate of bacterial over-infection was observed in the two AKI groups compared with the patients without AKI, suggesting that the sustained inflammation and its possible role in AKI persistence may not be due to bacterial over-infection.
A sustained inflammatory response and consequent coagulation activation may play a role in the development and persistence of COVID-19-related AKI.The SARS-Cov2 virus causes a dysregulation of the immune response leading to cytokine storm and macrophage activation syndrome.In this context, the complement activation stimulates coagulation pathways [38].Inflammation and coagulation stimulate each other and may expose the kidney to intensive and repeated stimuli.Furthermore, glomerular and peritubular capillaries obstruction, due to erythrocyte aggregation and fibrin thrombi, has suggested a role of kidney thrombotic microangiopathy in COVID-19-related AKI pathophysiology [20,21].The significant effect of the AKI group on d-dimer, along with a higher level of d-dimer observed in the persistent AKI group when compared with the no AKI group on the first day of ICU may support this pathological mechanism in our population.
Despite no difference in the severity of hypoxemic respiratory failure (i.e., similar PaO 2 /FiO 2 ratio and respiratory system mechanics at ICU admission) among the subgroups, an increased need for higher MV at ICU admission was required through the AKI spectrum.Furthermore, a higher MV was also required in the persistent AKI cohort during the following days until day 7.When considering the average respiratory parameters during the first week of ICU, we observed a significant trend towards a lower EtCO 2 and a higher estimated dead space among the three groups.We noted a significantly higher percentage of subjects developing persistent AKI in the higher MV and standardized MV quartiles with a trend toward a higher percentage of persistent AKI in the quartiles with more hypercapnic patients.Sustained hypercapnia was not associated with an increase in serum creatinine in non-COVID-19 ARDS [39].However, our findings are consistent with previous reports about critically ill COVID-19 and ARDS patients, where an increased dead space [40,41] and a significant impairment in CO 2 clearance [39] were described.
We were not able to support the perfusion deficit as the leading cause of increased dead space through autopsy evaluations or imaging techniques.However, previous autopsy data reported inflammatory infiltration of endothelial cells and micro-thrombosis in the lung tissue of COVID-19 patients [42].
In our cohort, standardized MV is independently associated with the onset and persistence of AKI after adjusting for confounding factors.Although standardized MV does not directly measure alveolar dead space, it can be considered a strong proxy of dead space [30], as it effectively characterizes the additional ventilatory load required to maintain PaCO 2 within the normal range.An increase in standardized MV has been observed with the severity of ARDS [5].
The absence of association between bicarbonate with AKI in the multivariable model suggests that the increase in the MV is probably aimed at keeping acceptable CO 2 levels because of higher dead space in the presence of AKI.Even if our hypothesis needs to be further investigated from a histologic and imaging point of view, our preliminary findings suggest that a perfusion deficit may be a common mechanism in the development of lung and kidney failure.
The proportion of patients who underwent prone positioning decreased from the no-AKI to the persistent AKI group.Furthermore, patients who underwent prone positioning were ventilated with higher PEEP levels, than those who did not, which may be explained by a higher severity of respiratory failure in patients undergoing prone position.The benefits related to a more homogeneous ventilation and distribution of total stress and strain, which leads to a reduced risk of VILI [43,44], may have a protective role against AKI development and persistence by improving lung compliance and gas exchange and reducing the systemic effect of biotrauma [16,17].This finding underscores the crucial role of personalized PEEP titration [45] and positional therapy in ARDS [46,47], as well as the need to combine lung and kidney protection.Recently, Fogagnolo and colleagues proposed the Renal Resistive Index (RRI) as a promising non-invasive diagnostic tool to predict the risk of AKI in mechanically ventilated patients and that the PEEP setting may contribute to modulate the resistance of the renal blood flow and be associated with the onset of AKI [48].
We observed a trend toward more severe hemodynamic impairment in patients with persistent AKI.We can suppose that despite the attempt to ensure an adequate cardiac output through a compensatory increase of heart rate and a higher dose of vasoactive/inotropic drugs (i.e., similar mean arterial pressure among the group but higher cardiovascular SOFA score in AKI patients), the peripheral perfusion was suboptimal in patients who developed sustained AKI.Therefore, we cannot exclude that a reduction of renal blood flow may be a contributing factor to AKI development.
This study has some limitations.First, we based the AKI definition only on serum creatinine over 7 days from ICU admission, according to the 2012 KDIGO cutoff [25], because the hourly urinary output was not available on the clinical charts.This may lead to an underestimation of the AKI cohort in our population [49].Furthermore, given the well-known limitations of serum creatinine in the assessment and prognostication of AKI, new biomarkers of kidney injury may offer further insights into characterizing COVID-19-associated AKI [50].Secondly, we estimated serum creatinine for the patients in which the baseline renal function was not available within six months prior to the ICU admission.Even if the 2021 CKD-EPI creatinine formula [28] has been demonstrated to be accurate, some misclassifications may occur.Finally, no histological findings were available in our research to prove the potential common mechanism of increased dead space and kidney injury from a biological standpoint.

Conclusions
In conclusion, in a population of critically ill patients with COVID-19 respiratory failure requiring ICU admission and mechanical ventilation, a high rate of wasted ventilation and dead space is linked to a higher risk of developing persistent AKI.Our finding generated the hypothesis that acute kidney and lung injury may be driven by common and chained pathophysiologic processes within a lung-kidney injury cross-talk.The concept of alveolar dead space, as ventilated but non-perfused parenchyma, in the presence of significant systemic inflammatory activation, may be applied to other organs.In this scenario, various pathophysiologic mechanisms in COVID-19-related ARDS-such as vascular thromboembolism and vasoconstriction-could impair renal perfusion and filtration, resulting in a shared scenario of lung and "renal dead space".

Fig. 1
Fig. 1 Creatinine, d-dimer, WBC, and PCT during the first ICU week, stratified in no AKI, transient AKI, and persistent AKI groups.The trend in creatinine, d-dimer, WBC, and PCT over time in patients without AKI (green circles), with transient (blue triangles), and persistent (red squares) AKI.All data represent mean ± SEM. $ refers to the effect of AKI groups on the continuous variable; $$ refers to the effect of day from ICU admission on the continuous variable; $$$ refers to the interaction between AKI groups and day from ICU admission on the continuous variable.

✚P < 0 .
05 for comparison between no AKI and transient AKI groups; *P < 0.05 for comparison between no-AKI and persistent AKI groups; # P < 0.05 for comparison between transient and persistent AKI groups.Definition of abbreviation.PCT, procalcitonin; WBC, white blood cells

aFig. 2 Fig. 3 Fig. 4
Fig. 2 PaCO 2 , minute ventilation, and standardized minute ventilation, during the first ICU week, stratified in no AKI, transient AKI, and persistent AKI groups.The trend in PaCO 2 , minute ventilation, and standardized minute ventilation over time in patients without AKI (green circles), with transient (blue triangles), and persistent (red squares) AKI.All data represent mean ± SEM.$ refers to the effect of AKI groups on the continuous variable; $$ refers to the effect of day from ICU admission on the continuous variable; $$$ refers to the interaction between AKI groups and day from ICU admission on the continuous variable.✚ P < 0.05 for comparison between no-AKI and transient AKI groups; *P < 0.05 for comparison between no-AKI and persistent AKI groups; # P < 0.05 for comparison between transient and persistent AKI groups.Definition of abbreviation.PaCO 2 , arterial partial pressure of carbon dioxide

Table 1
Baseline characteristics and renal characterization of the study populationContinuous data will be expressed as mean ± SD or median [IQR], as appropriate; categorical variables were reported as count (proportion) (n, %) Definition of abbreviation.BMI Body mass index, COPD Chronic obstructive pulmonary disease, CRRT Continuous renal replacement therapy, ICU Intensive care unit, OSAS Obstructive sleep apnea syndrome * P values for trend were calculated with the use of linear regression, Jonckheere-Terpstra, and Cochran-Armitage trend test, where appropriate a Nephrotoxic drugs included non-steroidal anti-inflammatory drugs (NSAIDs), Colistin, Aminoglycosides, and Vancomycin first 7 ICU days was lower in AKI patients.Patients were ventilated with higher median PEEP and FiO 2 levels in the first week of ICU stay by moving through AKI classes.

Table 2
Laboratory, ventilatory, and hemodynamic parameters during ICU stay

Table 2
(continued) Continuous data will be expressed as mean ± SD or median [IQR], as appropriate; categorical variables were reported as count (proportion) (n, %) Definition of abbreviation.ALT Alanine transaminase, aPTT Activated partial thromboplastin time, AST aspartate Aminotransferase, CRP C-reactive protein, CVP Central venous pressure, Cpl,rs Compliance of respiratory system, DP Driving pressure, EtCO 2 End-tidal CO 2 , FiO 2 Inspiratory fraction of oxygen, HR Heart rate, IBW Ideal body weight, ICU LOS Intensive care unit length of stay, IMV Invasive mechanical ventilation, iNO Inhaled nitric oxide, INR International normalized ratio, LDH Lactate dehydrogenase, MAP Mean arterial pressure, MV Minute ventilation, NIMV Non-invasive mechanical ventilation, PaCO 2 Arterial partial pressure of carbon dioxide, PaO 2 Arterial partial pressure of oxygen, PCT Procalcitonin, PEEP Positive end-expiratory pressure, PTE Pulmonary thromboembolism, RR Respiratory rate, SOFA Sequential organ failure assessment, TV Tidal volume, VFDs Ventilator-free days, VTE Venous thromboembolism, V-V ECMO Veno-venous extracorporeal membrane oxygenation, WBC White blood cells * P values for trend were calculated with the use of linear regression, Jonckheere-Terpstra, and Cochran-Armitage trend test, where appropriate † Chi 2 test was used to estimate differences in the proportions of renal recovery between transient and persistent AKI groups

Table 3
Ordered multivariable analysis of factors independently associated with the probability of development and persistence of AKI MV Minute ventilation, SOFA Sequential organ failure assessment a Standardized minute ventilation = minute ventilation × PaCO 2 /40 mm Hg